INVESTIGATION OF GENETIC DIVERSITY OF CONVOLVULUS ARVENSIS L. APPLYING ISSR MOLECULAR MARKERS

Abstract

Genetic diversity assessment enables plant breeders and genetic researchers to conserve unique plant genomes. Conservation of sole genetic characteristics enables plants to endure environmental fluctuations in various habitats. The main objective of this research was to investigate the genetic variation in Convolvulus arvensis L. (Bindweed) with phenotypic variant flower colors. Genomic DNA was extracted through the CTAB method from five samples each (white and pink). ISSR (Inter Simple Sequence Repeats) molecular markers (UBC-825 and UBC-835) were applied to assess genetic assortment. The in-formativeness of the ISSR markers (polymorphism information content, allelic frequency, and genetic diversity) were analyzed and the dendrogram was constructed by using power markers 3.25. Five pink and two white flower Convolvulus arvensis L. samples produce amplification products in ISSR PCR analysis. UBC-835 produces polymorphic bands for both samples. The size of amplicon for pink flower and white flower samples ranges from 200bp-500bp. The in-formativeness of the ISSR marker was 50% polymorphism in samples, the polymorphism information content (PIC) value for markers was 0.1371, the mean value of allelic frequency was 0.9158, and the mean value of genetic diversity in variant samples were 0.1507 observed. Power marker (3.25) and Mega software were used for the construction of the dendrogram. Two different flower colors of bindweed may be due to influences of biotic and abiotic factors. ISSR (UBC-825 and UBC-835) molecular marker indicated limited genetic diversity within bindweed and helped analyze possible environmental fluctuations.